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Multiplex PCR Design

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MPD - Multiplex PCR Design

by Thomas Wingo and David Cutler


MPD is a program designed to automate creation of multiplex primer design written in C. The mpd_lessGreedy and mpd_moreGreedy binaries differ in which primer pool they choose to start with for pool creation. Either binary can be used as stand-alone or in conjunction with the MPD perl package.


Required files

Build Hashed Genome

Flat dbSnp

name numberOfReporters chrom position MinorAlleleFrequency allele1/allele2

Run mpd